De novo Sequencing of Proteins With Mass Spectrometry Using the Differential Scanning Technique

peer-reviewed · Proteome and Protein Analysis · 2011

peer-reviewed · Proteome and Protein Analysis · 2011. Matthias Wilm et al.
Date 2011-09-11
Type peer-reviewed
Venue Proteome and Protein Analysis
Publisher Springer Berlin Heidelberg
Contribution algorithm
DOI 10.1007/978-3-642-59631-5_5
Citations (OpenAlex) 6

Authors

  1. Matthias Wilm · European Molecular Biology Laboratory, Max Planck Institute of Molecular Cell Biology and Genetics
  2. Gitte Neubauer · European Molecular Biology Laboratory
  3. L. Taylor
  4. Andrej Shevchenko · European Molecular Biology Laboratory, Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society, University of California San Diego
  5. A. Bachi · European Molecular Biology Laboratory

Methods and tools

  • Differential scanning de novo sequencing: Resolves the ion-type ambiguity that makes a tandem mass spectrum hard to read by acquiring the same peptide under differing conditions and comparing the scans, so a fragment’s series can be assigned rather than inferred. Aimed at organisms whose genomes are not sequenced, where a database is not an option.

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