EThcD as a Unique Tool for the Top-Down De Novo Sequencing of Intact Natural Ranid Amphibian Peptides
peer-reviewed · Analytical Chemistry · 2024
| Date | 2024-07-23 |
| Type | peer-reviewed |
| Venue | Analytical Chemistry |
| Publisher | American Chemical Society (ACS) |
| Contribution | downstream-application |
| DOI | 10.1021/acs.analchem.4c02109 |
| Citations (OpenAlex) | 7 |
| Venue 2-year citedness | 7.10 |
Abstract
De novo sequencing of any novel peptide/protein is a difficult task. Full sequence coverage, isomeric amino acid residues, inter- and intramolecular S-S bonds, and numerous other post-translational modifications make the investigators employ various chemical modifications, providing a variety of specific fragmentation MS n patterns. The chemical processes are time-consuming, and their yields never reach 100%, while the subsequent purification often leads to the loss of minor components of the initial peptide mixture. Here, we present the advantages of the EThcD method that enables establishing the full sequence of natural intact peptides of ranid frogs in de novo top-down mode without any chemical modifications. The method provides complete sequence coverage, including the cyclic disulfide section, and reliable identification of isomeric leucine/isoleucine residues. The proposed approach demonstrated its efficiency in the analysis of peptidomes of ranid frogs from several populations of Rana arvalis , Rana temporaria , and Pelophylax esculentus complexes.
Methods and tools
- EThcD top-down ranid peptide sequencing: Top-down de novo sequencing of intact ranid frog skin peptides by EThcD, giving full sequence coverage including the disulfide ring, and telling leucine from isoleucine, without chemical modification.
Cites (6)
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