Oxidation versus carboxamidomethylation of s-s bond in ranid frog peptides: Pro and contra for de novo MALDI-MS sequencing
peer-reviewed · Journal of the American Society for Mass Spectrometry · 2008
| Date | 2008-04-01 |
| Type | peer-reviewed |
| Venue | Journal of the American Society for Mass Spectrometry |
| Publisher | American Chemical Society (ACS) |
| Contribution | adjacent |
| DOI | 10.1016/j.jasms.2007.12.010 |
| Citations (OpenAlex) | 32 |
| Venue 2-year citedness | 2.84 |
Abstract
Five natural peptides isolated from ranid skin secretions of European frog species of Rana ridibunda and Rana arvalis (molecular masses 3516, 2674, 2636, 1874, and 1810 Da) were studied by MALDI-TOF/TOF to compare two procedures of disulfide bond cleavage: (1) performic oxidation and (2) reduction/carboxamidomethylation. The processes are relevant for the elucidation of the amino acid sequence inside the seven-member cystine ring at the C-terminus. The results clearly demonstrated that oxidation of the disulfide bond led to notably higher abundances of b- and y-ions, corresponding to the C-terminal peptide bonds, than reduction/carboxamidomethylation. This conclusion is true for all five peptides studied. Besides that, the oxidation procedure is simpler than carboxamidomethylation, as it is a one-step process with no purification required. The oxidation is more reproducible. The results were similar each time the peptide was subjected to the process. It was successfully applied to all five peptides while reduction/carboxamidomethylation failed in the case of brevinin-1Ra, despite all variations of reaction conditions.
Methods and tools
- Disulfide oxidation for de novo MALDI sequencing: Shows that performic acid oxidation of the C-terminal cystine ring in frog skin peptides gives more b and y ions than reduction and alkylation, making MALDI de novo sequencing easier.
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- Modern Approaches in de novo Sequencing of Nontryptic Peptides of Ranid and Hylid Frogs by Means of Mass Spectrometry: A Review (2022) crossref
- FT‐MS in the de novo top‐down sequencing of natural nontryptic peptides (2022) crossref
- Isolation and sequence analysis of peptides from the skin secretion of the Middle East tree frog Hyla savignyi (2010) crossref
- N-terminal tagging strategy forDe Novosequencing of short peptides by ESI-MS/MS and MALDI-MS/MS (2010) both
- De novo sequencing of peptides secreted by the skin glands of the Caucasian Green Frog Rana ridibunda (2008) crossref