Mass spectrometry-assisted venom profiling of Hypnale hypnale found in the Western Ghats of India incorporating de novo sequencing approaches

peer-reviewed · International Journal of Biological Macromolecules · 2018

peer-reviewed · International Journal of Biological Macromolecules · 2018. Muralidharan Vanuopadath et al.
Date 2018-07-07
Type peer-reviewed
Venue International Journal of Biological Macromolecules
Publisher Elsevier
Contribution downstream-application
DOI 10.1016/j.ijbiomac.2018.07.016
Citations (OpenAlex) 27

Authors

  1. Muralidharan Vanuopadath · Amrita Vishwa Vidyapeetham
  2. Nithin Sajeev · Amrita Vishwa Vidyapeetham
  3. Athira Radhamony Murali · Amrita Vishwa Vidyapeetham
  4. Nayana Sudish · Amrita Vishwa Vidyapeetham
  5. Nithya Kangosseri · Amrita Vishwa Vidyapeetham
  6. Ivy Rose Sebastian · Amrita Vishwa Vidyapeetham
  7. Nidhi Dalpatraj Jain · Amrita Vishwa Vidyapeetham
  8. Amit Pal · Amrita Vishwa Vidyapeetham
  9. Dileepkumar Raveendran · Indriyam Biologics Pvt. Ltd., University of Kerala
  10. Bipin Gopalakrishnan Nair · Amrita Vishwa Vidyapeetham
  11. Sudarslal Sadasivan Nair · Amrita Vishwa Vidyapeetham

Methods and tools

  • Hypnale hypnale venomics: Venomics workflow combining SDS-PAGE + LC-MS/MS with Novor + PEAKS de novo sequencing and MS-BLAST similarity search to characterize the venom proteome of the hump-nosed pit viper (Western Ghats).
  • MS BLAST validation: Validation of borderline protein identifications using de novo sequence tags and MS BLAST searches.
  • Novor: Real-time decision-tree scoring
  • PEAKS: Commercial DP-based de novo

Cites (3)

Cited by (2)

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