Mass spectrometry-assisted venom profiling of Hypnale hypnale found in the Western Ghats of India incorporating de novo sequencing approaches
peer-reviewed · International Journal of Biological Macromolecules · 2018
| Date | 2018-07-07 |
| Type | peer-reviewed |
| Venue | International Journal of Biological Macromolecules |
| Publisher | Elsevier BV |
| Contribution | downstream-application |
| DOI | 10.1016/j.ijbiomac.2018.07.016 |
| Citations (OpenAlex) | 28 |
| Venue 2-year citedness | 8.99 |
Abstract
Hypnale hypnale (hump-nosed pit viper) is considered to be one among the medically important venomous snake species of India and Sri Lanka. In the present study, venom proteome profiling of a single Hypnale hypnale from Western Ghats of India was achieved using SDS-PAGE based protein separation followed by LC-MS/MS analysis. The identities of the proteins that were not established using the Mascot search were determined through de novo sequencing tools such as Novor followed by MS-BLAST based sequence similarity search algorithm and PEAKS proteomics software. The combined proteomics analysis revealed a total of 37 proteins belonging to nine different snake venom families, in which 7 proteins were exclusively identified through de novo strategies. The enzymatic and non-enzymatic venom protein families identified include serine proteases, metalloproteases, phospholipase A 2 , thrombin-like enzymes, phospholipase B, C-type lectins/snaclecs, disintegrins, cysteine rich secretory proteins and nerve growth factor. Among these, disintegrins, nerve growth factor, phospholipase B and cysteine rich secretory protein families were identified for the first time in HPV venom. This could possibly explain the regiospecific venom variation seen across snake species. Taken together, the venom proteome profiling on Indian Hypnale hypnale venom correlates with the clinical manifestations often seen in the envenomed victims.
Methods and tools
- Hypnale hypnale venomics: Venomics workflow combining SDS-PAGE + LC-MS/MS with Novor + PEAKS de novo sequencing and MS-BLAST similarity search to characterize the venom proteome of the hump-nosed pit viper (Western Ghats).
Methods it uses
Data deposited
- Proteomics of Hypnale hypnale (Hump-nosed pit viper) venom from the Western Ghats of India (as deposited) · PXD017592
Cites (6)
- DeNovoGUI: An Open Source Graphical User Interface for de Novo Sequencing of Tandem Mass Spectra (2014) crossref
- PEAKS DB: De Novo Sequencing Assisted Database Search for Sensitive and Accurate Peptide Identification (2012) crossref
- Sequence similarity‐driven proteomics in organisms with unknown genomes by LC‐MS/MS and automated de novo sequencing (2007) crossref
- De novo protein sequence analysis of Macaca mulatta (2007) crossref
- De novo sequencing of peptides using MALDI/TOF-TOF (2002) crossref
- Charting the Proteomes of Organisms with Unsequenced Genomes by MALDI-Quadrupole Time-of-Flight Mass Spectrometry and BLAST Homology Searching (2001) crossref
Cited by (2)
- Venomics and antivenomics of Indian spectacled cobra (Naja naja) from the Western Ghats (2022) crossref
- Delineating the venom toxin arsenal of Malabar pit viper (Trimeresurus malabaricus) from the Western Ghats of India and evaluating its immunological cross-reactivity and in vitro cytotoxicity (2020) crossref