Spectrum Fusion: Using Multiple Mass Spectra for De Novo Peptide Sequencing
peer-reviewed · Journal of Computational Biology · 2009
peer-reviewed · Journal of Computational Biology · 2009. Ritendra Datta et al. We report on a new algorithm for combining the information from several mass spectra of the same peptide. The…
| Date | 2009-08-01 |
| Type | peer-reviewed |
| Venue | Journal of Computational Biology |
| Publisher | SAGE Publications |
| Contribution | algorithm |
| DOI | 10.1089/cmb.2009.0122 |
| Citations (OpenAlex) | 41 |
| Venue 2-year citedness | 1.54 |
Abstract
We report on a new algorithm for combining the information from several mass spectra of the same peptide. The algorithm automatically learns peptide fragmentation patterns, so that it can handle spectra from any instrument and fragmentation technique. We demonstrate the utility of the algorithm, and the power of multiple spectra, by showing that combining pairs of spectra (one CID and one ETD) greatly improves de novo sequencing success rates.
Methods and tools
- Spectrum Fusion: Combines several tandem mass spectra of the same peptide, acquired under different conditions or charge states, into one fused spectrum before de novo sequencing, which raises the success rate over sequencing any single spectrum.
Cites (14)
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Cited by (22)
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- De Novo Sequencing of Proteins and Peptides: Algorithms, Applications, Perspectives (2018) crossref
- Antibody de novo Sequencing (2017) crossref
- Comprehensive de Novo Peptide Sequencing from MS/MS Pairs Generated through Complementary Collision Induced Dissociation and 351 nm Ultraviolet Photodissociation (2017) both
- De Novo Sequencing of Top-Down Tandem Mass Spectra: A Next Step towards Retrieving a Complete Protein Sequence (2017) semanticscholar
- De Novo MS/MS Sequencing of Native Human Antibodies (2017) both
- De novo Peptide Sequencing (2016) crossref
- UVnovo: A de Novo Sequencing Algorithm Using Single Series of Fragment Ions via Chromophore Tagging and 351 nm Ultraviolet Photodissociation Mass Spectrometry (2016) both
- Application of de Novo Sequencing to Large-Scale Complex Proteomics Data Sets (2016) crossref
- Lessons in de novo peptide sequencing by tandem mass spectrometry (2015) both
- UniNovo: a universal tool for de novo peptide sequencing (2013) both
- Sequencing-Grade De novo Analysis of MS/MS Triplets (CID/HCD/ETD) From Overlapping Peptides (2013) both
- pNovo+: De Novo Peptide Sequencing Using Complementary HCD and ETD Tandem Mass Spectra (2013) crossref
- Constrained De Novo Sequencing of Conotoxins (2012) both
- Antilope—A Lagrangian Relaxation Approach to the de novo Peptide Sequencing Problem (2012) both
- De Novo Sequencing and Homology Searching (2012) both
- Database independent proteomics analysis of the ostrich and human proteome (2012) crossref
- Algorithms for the de novo sequencing of peptides from tandem mass spectra (2011) crossref
- Constrained De Novo Sequencing of Peptides with Application to Conotoxins (2011) crossref
- Algorithm Development of de novo Peptide Sequencing Via Tandem Mass Spectrometry (2010) crossref
- pNovo: De novo Peptide Sequencing and Identification Using HCD Spectra (2010) crossref