Fast algorithm for peptide sequencing by mass spectroscopy

peer-reviewed · Biological Mass Spectrometry · 1990

peer-reviewed · Biological Mass Spectrometry · 1990. Christian Bartels. An automatic algorithm for sequencing polypeptides from fast atom bombardment tandem mass spectra is…
Date 1990-06-01
Type peer-reviewed
Venue Biological Mass Spectrometry
Publisher Wiley
Contribution algorithm
DOI 10.1002/bms.1200190607
Citations (OpenAlex) 129
Venue 2-year citedness 0.00

Abstract

An automatic algorithm for sequencing polypeptides from fast atom bombardment tandem mass spectra is presented.Based on graph theory considerations it finds the most probable sequences, even if the amino acid composition is unknown, by scoring mass differences. The algorithm is fast as the computing time increases by less than the square of the number of amino acids. Pairs of two or three amino acids are proposed to explain the gap if peaks are missing.

Authors

  1. Christian Bartels · University of Basel

Methods and tools

  • Fast peptide sequencing algorithm (Bartels): 1990 graph-theory algorithm that sequences polypeptides from FAB tandem mass spectra by scoring mass differences, without needing the amino acid composition, and proposes amino acid pairs or triples to bridge missing peaks. Sub-quadratic in the number of residues.

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