Sequence tag
5 methods · 1994–2008
Sequence tag: Short, high-confidence stretches of sequence read straight off the spectrum and used as a filter or a query rather than as the final answer. A tag plus its flanking masses can identify a peptide on its own.
Short, high-confidence stretches of sequence read straight off the spectrum and used as a filter or a query rather than as the final answer. A tag plus its flanking masses can identify a peptide on its own.
The earliest of its 5 methods is Peptide sequence tags (1994); 4 more have followed.
| Methods | 5 |
| Papers describing them | 5 |
| Authors | 22 |
| Active | 1994-12-15 to 2008-09-05 |
| Kinds | adjacent (5) |
| Acquisition | DDA (5) |
Methods (5)
Oldest first, by the paper that describes each one.
- Peptide sequence tags (1994): Error-tolerant peptide identification using short de novo sequence tags and flanking masses.
- MultiTag (2003): Searches several error-tolerant peptide sequence tags at once, so a protein can be identified across species by sequence similarity even when no tag is individually correct. Tolerating errors in the tags is what lets de novo output drive a homology search.
- GutenTag (2003): High-throughput sequence tagging using an empirically derived fragmentation model.
- InsPecT (2005): Uses peptide sequence tags derived from the spectrum as a database filter, discarding the vast majority of candidate peptides before scoring, which makes blind searches for post-translational modifications tractable.
- DirecTag (2008): Open-source statistical scoring algorithm for accurate sequence tags from peptide MS/MS spectra.
Papers describing them (5)
- Error-Tolerant Identification of Peptides in Sequence Databases by Peptide Sequence Tags (1994, Analytical Chemistry, peer-reviewed)
- MultiTag: Multiple Error-Tolerant Sequence Tag Search for the Sequence-Similarity Identification of Proteins by Mass Spectrometry (2003, Analytical Chemistry, peer-reviewed)
- GutenTag: High-Throughput Sequence Tagging via an Empirically Derived Fragmentation Model (2003, Analytical Chemistry, peer-reviewed)
- InsPecT: Identification of Posttranslationally Modified Peptides from Tandem Mass Spectra (2005, Analytical Chemistry, peer-reviewed)
- DirecTag: Accurate Sequence Tags from Peptide MS/MS through Statistical Scoring (2008, Journal of Proteome Research, peer-reviewed)