Application of Top-Down Deconvolution Tools to Bottom-Up Mass Spectrometry Data: Advantages and Limitations

peer-reviewed · Springer Proceedings in Physics · 2022

peer-reviewed · Springer Proceedings in Physics · 2022. Kirill Tyshuk et al.
Date 2022-01-12
Type peer-reviewed
Venue Springer Proceedings in Physics
Publisher Springer International Publishing
Contribution algorithm
DOI 10.1007/978-3-030-81119-8_13
Citations (OpenAlex) 1

Authors

  1. Kirill Tyshuk · St Petersburg University
  2. Kira Vyatkina · ITMO University, Saint Petersburg Academic University, Saint Petersburg National Research Academic University of the Russian Academy of Sciences, Saint Petersburg State Electrotechnical University, St Petersburg University, St. Petersburg State University

Methods and tools

  • T-Bruijn graph top-down de novo sequencing: De novo sequencing from TOP-DOWN spectra, where the whole protein is fragmented rather than tryptic peptides: derives sequence tags and assembles them through a T-Bruijn graph, evaluated on carbonic anhydrase 2 and an alemtuzumab Fab.

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