Direct Protein Identification from Nonspecific Peptide Pools by High‐Accuracy MS Data Filtering

peer-reviewed · Angewandte Chemie International Edition · 2006

peer-reviewed · Angewandte Chemie International Edition · 2006. Vinh An Thieu et al. Validate your results! High-accuracy mass spectrometric analysis and composition-based de novo sequencing…
Date 2006-05-12
Type peer-reviewed
Venue Angewandte Chemie International Edition
Publisher Wiley
Contribution algorithm
DOI 10.1002/anie.200503787
Citations (OpenAlex) 20

Abstract

Validate your results! High-accuracy mass spectrometric analysis and composition-based de novo sequencing (CBS) combined with database(DB)-assisted protein identification allows for the rapid production of validated analytical results in proteome research. A simplified sample preparation scheme is sufficient to provide for reliable identification of proteins even when following nonspecific and unknown degradation processes.

Authors

  1. Vinh An Thieu · Justus-Liebig-Universität Gießen
  2. Dieter Kirsch · Justus-Liebig-Universität Gießen
  3. Thomas Flad · University of Tübingen
  4. Claudia Müller · University of Tübingen
  5. Bernhard Spengler · Justus Liebig University of Giessen, Justus-Liebig-Universität Gießen

Methods and tools

  • Composition-based sequencing: Two-step database-free strategy enabled by FT-ICR mass accuracy: determine the peptide’s amino acid COMPOSITION first, then score only the permutations that composition allows, which is a far smaller search space than conventional de novo sequencing explores.

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