Simplified validation of borderline hits of database searches
peer-reviewed · PROTEOMICS · 2008
peer-reviewed · PROTEOMICS · 2008. Henrik Thomas et al. Along with unequivocal hits produced by matching multiple MS/MS spectra to database sequences, LC-MS/MS…
| Date | 2008-10-01 |
| Type | peer-reviewed |
| Venue | PROTEOMICS |
| Publisher | Wiley |
| Contribution | adjacent |
| DOI | 10.1002/pmic.200800250 |
| Citations (OpenAlex) | 7 |
Abstract
Along with unequivocal hits produced by matching multiple MS/MS spectra to database sequences, LC-MS/MS analysis often yields a large number of hits of borderline statistical confidence. To simplify their validation, we propose to use rapid de novo interpretation of all acquired MS/MS spectra and, with the help of a simple software tool, display the candidate sequences together with each database search hit. We demonstrate that comparing hit database sequences and independent de novo interpretations of the same MS/MS spectra assists in rapid examination of ambiguous matches.
Methods and tools
- De novo cross-check of borderline database hits: Displays rapid de novo interpretations next to each borderline database search hit so ambiguous matches can be checked by eye.