Spectral alignment / assembly
2 methods · 2009–2013
Spectral alignment / assembly: Reads a peptide from the relationships between spectra rather than from one spectrum alone, aligning or assembling overlapping acquisitions so that evidence missing from any single fragmentation is supplied by another.
Reads a peptide from the relationships between spectra rather than from one spectrum alone, aligning or assembling overlapping acquisitions so that evidence missing from any single fragmentation is supplied by another.
The earliest of its 2 methods is NRP dereplication and sequencing (2009).
| Methods | 2 |
| Papers describing them | 2 |
| Authors | 12 |
| Active | 2009-08-01 to 2013-06-07 |
| Kinds | algorithm (2) |
| Acquisition | DDA (2) |
Methods (2)
Oldest first, by the paper that describes each one.
- NRP dereplication and sequencing (2009): Sequences cyclic nonribosomal peptides from multistage mass spectrometry by spectral alignment, and dereplicates them comparatively against known NRPs. The first high-throughput route to a compound class with no genome to search and considerable pharmacological interest.
- MS/MS Triplets (2013): Sequencing-grade de novo analysis from overlapping CID/HCD/ETD MS/MS triplets.
Papers describing them (2)
- Dereplication and de novo sequencing of nonribosomal peptides (2009, Nature Methods, peer-reviewed)
- Sequencing-Grade De novo Analysis of MS/MS Triplets (CID/HCD/ETD) From Overlapping Peptides (2013, Journal of Proteome Research, peer-reviewed)