Venomics

50 workflows · 2004–2026

Venomics: Venom from snakes, spiders, scorpions, ants and cone snails, where the species has no sequenced genome and the toxins are heavily modified. 50 catalogued workflows and 50 papers.

Venom from snakes, spiders, scorpions, ants and cone snails, where the species has no sequenced genome and the toxins are heavily modified.

Workflows 50
Papers 50
Authors 279
Active 2004-02-01 to 2026-04-24

Workflows (50)

  • Wolf spider venom AMPs (lycocitins) (2004): De novo sequences the antimicrobial peptides lycocitin 1, 2 and 3 from the venom glands of the wolf spider Lycosa singoriensis, two of them new, alongside an unassigned 3038.70 Da peptide.
  • Atractaspis irregularis sarafotoxins (2005): Direct FTMS analysis of crude Atractaspis irregularis venom, with de novo sequencing confirming two new sarafotoxin families and characterizing a third, combined with precursor cloning.
  • Pseudonaja textilis venom diversity (2006): Profiles the venom of the Australian eastern brown snake by 2-DE, MS and de novo sequencing, finding new venom proteins and their modifications.
  • Conus virgo T-superfamily conotoxins (2006): Direct FTMS analysis of crude Conus virgo venom with disulfide reduction allowed de novo sequencing of two new modified T-superfamily conotoxins.
  • Bromotryptophan conotoxin sequencing (2006): Sequences a T-1-family conotoxin de novo by FTICR and maps its disulfides, a class whose 10 to 17 residues carry unusual modifications including brominated tryptophan that defeat database identification.
  • Australian elapid snake venom proteomes (2007): 2-DE and de novo peptide sequencing catalogue venom proteins of 18 Australian elapid species, including several novel protein families.
  • Cane toad parotid secretion peptides (2011): De novo sequencing of peptides in the parotid secretion of the cane toad Bufo marinus, a highly toxic secretion whose peptide content, unlike amphibian skin, had gone unexamined.
  • Micrurus tener toxin partial de novo sequencing (2012): Manual and automated de novo sequencing with CID and ETD data recovered the N-terminal half of a disulfide-bridged Texas coral snake toxin.
  • Araneus ventricosus venomics (2013): Manual de novo sequencing combined with a cDNA-derived local database identified 130 toxin-like proteins in a spider venom and found methyl-esterified glutamate.
  • Tityus serrulatus venom PTM toxins (2013): Characterises Tityus serrulatus scorpion venom, including phospho- and glycoproteome, combining database search with de novo sequencing to reveal modified toxins.
  • Spider venom peptidome (SPITC + de novo) (2014): SPITC N-terminal derivatization + 18O labeling coupled with de novo peptide sequencing to simultaneously identify and quantify spider-venom peptides from species with unknown genomes.
  • Gila monster venom proteome (2015): De novo peptide sequencing of 2D-gel spots followed by homology-based identification characterises the gila monster venom proteome, 19 of its 39 proteins not reported before in helodermatid venom.
  • Indonesian king cobra top-down venomics (2015): Top-down and bottom-up venomics of Ophiophagus hannah, where de novo sequence tags from intact toxins identified three-finger toxins, a novel PLA2 and other proteins.
  • Bothriopsis taeniata PLA2 K49 (2015): Automatic de novo sequencing (PEAKS Studio) with CID/HCD/ETD triplex MS/MS to elucidate the full-length sequence of a new PLA2 K49 (Btt-TX) from Bothriopsis taeniata snake venom.
  • Acanthoscurria gomesiana venom peptidomics (2017): MS-based peptidomics of the Brazilian tarantula Acanthoscurria gomesiana venom, using de novo analysis plus transcriptome and homology searches to fully sequence mature cysteine-rich toxins and find new antimicrobial peptides.
  • Cone snail contryphans (2017): De novo sequencing of twelve short single-disulfide conopeptides from four cone snail species, including the shortest contryphan characterised and one with a brominated tryptophan.
  • Loxosceles intermedia venom proteome (2017): Multi-protease, multi-dissociation proteomics of brown spider venom, with spectral networks and meta-contig assembly yielding de novo sequences of nearly complete proteins.
  • Scorpion venom AMP sequencing (2017): Completes the sequences of antimicrobial peptides in Isometrus maculatus scorpion venom by mass spectrometry, where venom-gland transcriptomics had multiplied candidate AMPs without confirming the mature peptides.
  • Russell’s viper apyrase (Ruviapyrase) (2018): Purifies a novel apyrase from Daboia russelii venom whose identity rested on de novo sequences carrying apyrase domains.
  • Bufo gargarizans skin peptidome (2018): Peptidomics workflow using nano-LC-MS/MS and PEAKS de novo sequencing against a transcriptome-derived database to characterise <10 kDa bioactive peptides from Bufo gargarizans (Venenum Bufonis) skin secretions.
  • Hypnale hypnale venomics (2018): Venomics workflow combining SDS-PAGE + LC-MS/MS with Novor + PEAKS de novo sequencing and MS-BLAST similarity search to characterize the venom proteome of the hump-nosed pit viper (Western Ghats).
  • Tetramorium bicarinatum ant venom peptidome (2018): Venomics of the ant Tetramorium bicarinatum in which venom peptides were sequenced by de novo MS (PEAKS) or Edman and combined with transcriptomics to define 37 myrmicitoxin precursors in three superfamilies.
  • Ruby ant venom decapeptide (2019): Combined Edman degradation and de novo peptide sequencing to isolate and characterize U-MYRTX-MRArub1, a novel insecticidal decapeptide from Myrmica rubra ant venom.
  • Scorpion venom chemical-modification de novo (2019): Chemical modification-assisted de novo sequencing of antimicrobial peptides in scorpion venom.
  • Conus ateralbus conotoxin AtVIA (2019): Isolates and manually de novo sequences AtVIA, the first conotoxin from the Cabo Verde endemic cone snail Conus ateralbus, a 30-residue delta-conotoxin-like peptide that excites mouse dorsal root ganglion neurons.
  • Duttaphrynus melanostictus parotoid secretion proteins (2019): Proteins of the Asian common toad parotoid macrogland secretion were identified by database search and de novo analysis, the latter yielding 153 peptides.
  • Gyroxin structural variants (2019): De novo peptide sequencing showing structural variants of the gyroxin toxin in the venom of the rattlesnake Crotalus durissus terrificus.
  • Conus amadis conotoxin discovery (2019): Proteome-based de novo sequencing of novel conotoxins from the molluscivorous cone snail Conus amadis, with the neurological activity of the natural venom assayed in zebrafish.
  • Tetraponera aethiops ant venom peptidome (2019): Venom peptidome of the plant-ant Tetraponera aethiops from transcriptomics plus PEAKS de novo sequencing (ALC above 60%), characterising nine mature pseudomyrmecitoxin sequences, some dimeric.
  • Malabar pit viper venomics (2020): Venomics workflow combining SDS-PAGE and ion-exchange pre-fractionation with LC-MS/MS + Novor and PEAKS de novo sequencing to identify 97 proteins (including 18 exclusive to de novo) from Trimeresurus malabaricus venom, and evaluate antivenom cross-reactivity.
  • Anatolian meadow viper top-down ISD venomics (2020): Extended top-down venomics workflow using in-source decay (ISD) with 1,5-diaminonaphthalene reducing matrix to enable disulfide-bond counting and de novo sequencing-based identification of high-molecular-weight venom constituents, applied to the newly described Vipera anatolica senliki.
  • Manica rubida ant venom insecticidal toxins (2020): Venom peptidome of the red ant Manica rubida by transcriptomics plus PEAKS de novo peptide sequencing, identifying 13 myrmicitoxins, six of them insecticidal.
  • Conus zonatus single-disulfide conopeptide Czon1107 (2020): Identifies and de novo sequences single-disulfide conopeptides from Conus zonatus and Conus caracteristicus venom using MSn of native and derivatized peptides, then shows Czon1107 allosterically inhibits human nicotinic receptors.
  • Bothrops atrox venom peptidome (2020): Bottom-up and top-down peptidomics of male and female Bothrops atrox venoms that identified 105 native peptides, some sequenced de novo, and found higher disintegrin levels in female venoms.
  • Conus inscriptus conotoxins (2021): Manual de novo sequencing of eleven novel conotoxins from the venom of the vermivorous cone snail Conus inscriptus.
  • Buthacus leptochelys venom linear peptide (2021): De novo structure determination of a linear, disulfide-free peptide from the venom of the scorpion Buthacus leptochelys.
  • Naja naja venomics (2022): Venomics + antivenomics workflow using SDS-PAGE, RP-HPLC and Q-TOF LC-MS/MS with PEAKS + Novor de novo sequencing to identify 115 proteins across 17 venom protein families in Indian cobra venom from the Western Ghats, and benchmark polyvalent-antivenom recognition.
  • Hydrophis schistosus venomics (2022): Combined bottom-up proteomics with a transcriptome database and hybrid de novo sequencing to characterize beaked sea snake venom, the de novo step adding 46 toxin peptides.
  • Daboia siamensis multi-omics venomics (2022): Multi-omics venomics of Russell’s viper (Daboia siamensis, Thai specimens): 10x-linked-read + short-read genome assembly and venom-gland transcriptomics feed a MAKER-annotated CDS database, which is searched with PEAKS Studio (database) plus SMSNet (deep-learning de novo) to identify 1,662 venom proteins including previously unreported hyaluronidase-1, phospholipase B and waprin.
  • Bothrops moojeni venom protease de novo identification (2022): Reviews the state of identifying wholly unknown proteins by mass spectrometry and demonstrates it on a snake venom serine protease from Bothrops moojeni, where de novo sequence tags such as D[K/Q]D[I/L]VDD[K/Q] made the identification.
  • Myrmicine ant venom peptidomes (six species) (2022): Venom peptidomes of six myrmicine ants characterised by integrating transcriptomics with PEAKS de novo sequencing of venom peptides, defining 79 myrmicitoxin precursors in 38 families.
  • Batroxin I bradykinin-potentiating peptide (2023): Isolates a cytotoxic bradykinin-potentiating peptide from Bothrops atrox venom and determines its sequence by de novo MS/MS.
  • Snake venomics quantification review (2023): Calvete-lab review of mass-spectrometry-based quantification of snake venom proteomes for non-model species: label-free strategies, absolute quantification via ICP-MS, hybrid elemental/molecular MS setups. Discusses manual de novo interpretation as an established workflow for non-model venomics.
  • MELD snake venomics (2023): Applies multi-enzymatic limited digestion to four snake venoms, increasing de novo sequenced peptides and toxin coverage in PEAKS.
  • Lachesis muta venom peptidome (2023): Peptidomic survey of bushmaster (Lachesis muta) venom using PEAKS de novo sequencing with database and homology searches, reporting 151 peptides, 126 of them new, including the metalloproteinase-derived fragment Lm-10a.
  • Bothrops cotiara venom peptidome (Bc-7a) (2024): Venom peptidomics of Bothrops cotiara with PEAKS de novo sequencing, reporting 197 peptides and a new pyroglutamyl heptapeptide, Bc-7a, that inhibits angiotensin-converting enzyme.
  • Scorpion venom short peptides (2024): LC-HRMS with de novo sequencing of short (2-12 aa) scorpion venom peptides: 156 sequences identified, revealing N- and C-terminal modifications including the previously unreported N-benzoyl modification.
  • Spiderhawk wasp venom oligopeptides (2024): De novo sequencing of Pepsis decorata wasp venom identified 39 oligopeptides, including proctolin-like and bradykinin-related peptides.
  • Conus regius conopeptidome (2025): Reversed-phase LC + Q-TOF MS/MS with Peaks Studio v7 de novo sequencing to identify 520 conopeptides from the vermivorous cone snail Conus regius, spanning 13 conotoxin gene superfamilies and eight disulfide-poor peptide classes: the first molluscan entry in the venomics lane.
  • Honeybee venom TRPV1-targeting peptides (2026): PEAKS de novo sequencing of Apis mellifera venom produced a 472-peptide database, from which pull-down screening found ten novel TRPV1-binding peptides; three were synthesized and shown to be anti-inflammatory.

Papers (50)

Sequencing tools these papers used

MS BLAST, Manual MS/MS de novo interpretation, Novor, PEAKS, SMSNet, SPIDER

Authors (279)

A. K. Musolyamov, Adrian Guthals, Adriana Karaoglanovic Carmona, Adriana Simizo, Adriano M. C. Pimenta, Adélia Cristina Oliveira Cintra, Agostinho Antunes, Ahmed Hossam Mahmoud, Alain Dejean, Alan G. Marshall, Alexandre A. A. Dutra, Alexandre K. Tashima, Alexsandro Branco, Alica Klaus, Aline V. Bednaski, Amanda Almeida Resende, Amit Pal, Andrea Senff-Ribeiro, Andreas Vilcinskas, André Zelanis, Anita M. Tanaka-Azevedo, Anthony J. Saviola, Aparup Patra, Ashis K. Mukherjee, Athira Radhamony Murali, Atsushi Kitanaka, Axel Touchard, Ayse Nalbantsoy, B. A. Budnik, Baldomero M. Olivera, Bandar Mohsen Ahmed Almunqedhi, Bayram Göçmen, Beatrix Ueberheide, Benjamin Franklin Jayaseelan, Benjamin Lefranc, Benjamin-Florian Hempel, Bhargab Kalita, Bianca N. Sumitomo, Bipin Gopalakrishnan Nair, Bruno Lomonte, Cangman Zhang, Carlton Ranjith Wilson Alphonse, Carol L. Nilsson, Caroline Ligny-Lemaire, Chao Ji, Cheng Zhang, Christophe Klopp, Christopher J. Bohlen, Christopher Kune, Damien Redureau, Daniel C. Pimenta, Daniel Petras, David Morgenstern, Dileepkumar Raveendran, Dilza Trevisan-Silva, Dominique Baiwir, Douglas O. C. Mariano, Eduardo Shigueo Kitano, Elsa Bonnafé, Emídio Beraldo-Neto, Eric Haubruge, Eugene V. Grishin, Fabiana V. Campos, Fabricio K. Marchini, Fabíola Attié de Castro, Felipe V. Leprevost, Fernanda Gobbi Amorim, Francisco Calderón Celis, Frank Kjeldsen, Françoise Paquet, Frédéric Ducancel, Gabriel Mazzucchelli, Gabriel Neves Cezarette, Gauthier Eppe, Geoff W. Birrell, Gilles Phan, Graham M. Nicholson, Gustavo H.M.F. Souza, Hadrien Lalägue, Helena B. Fiorotti, Hisashi Miyagawa, Hongcheng Zhang, Hongyue Ma, Huixia Zhang, Humberto Fonseca de Freitas, Ida B. Thøgersen, Iffath Badsha, Ileana R. León, Inácio Junqueira-de-Azevedo, Ivy Rose Sebastian, Jackson G. Miyamoto, Jan J. Enghild, Jayaseelan B. Franklin, Jean-Pierre Le Caer, Jeffrey J. Gorman, Jesper V. Olsen, Jia Long, Jianfeng Qin, Jianfeng Sun, Jiangtao Qiao, Jie Dai, Jinao Duan, Jing Zhou, Jingguang Lu, Joanna Gajewiak, Johannes A. Eble, John Heep, John de Jersey, Jorge L. B. Neves, Jorge Ruiz Encinar, Joëlle Vinh, Juan J. Calvete, Julia Chamot-Rooke, Juliana S.G. Fischer, Julie Bourdais- Jomaro, Julita S. Imperial, Jérôme Leprince, Jérôme Orivel, K. S. Krishnan, Kai Wang, Katalin F. Medzihradszky, Kathleen F. Grego, Kenneth Hodge, Khin Than Yee, Konkallu Hanumae Gowd, Kristian W. Sanggaard, Lachlan D. Rash, Lars Brøndum, Laudicéia Alves De Oliveira, Laurent Coquet, Lawan Chanhome, Line R. Thomsen, Ling Zeng, Liping Jiang, Lotten Ragnarsson, Lou Freuville, Loïc Quinton, Lucas T. Ito, Lucilene Delazari Dos Santos, Luis Alberto Ponce-Soto, M.S. Vignesh, Madhan Kumar Mohan, Maik Damm, Mao Yakio, Marcela Di Giacomo Messias, Marcella N. Melo-Braga, Maren Watkins, Maria Luiza Vilela Oliva, Marisa Skaljac, Mark A. Knepper, Mark R. Emmett, Martin F. Lavin, Martin Kenne, Masahiro Miyashita, Masato Tanaka, Matheus Nolasco, Maurice Tindo, Maximilian Seip, Mei Zhou, Mert Karış, Michel Treilhou, Milton Y. Nishiyama, Mingrong Yang, Mohammed Abdel-Wahab, Montri Sumontha, Moustafa Sarhan, Mrinalini, Muralidharan Vanuopadath, Naixuan Cai, Nambali Valsalan Vinithkumar, Narongsak Chaiyabutr, Nathan Téné, Nayana Sudish, Nicholas R. Casewell, Nicolas Gilles, Nidhi Dalpatraj Jain, Nikita Abraham, Niklas Tysklind, Nithin Sajeev, Nithya Kangosseri, Nuno Bandeira, Nuttiya Kalpongnukul, Orawan Khow, Osama B. Mohammed, P. Arjun, Padmanabhan Balaram, Panithi Laoungbua, Patrick Jack Spencer, Patrícia Alessandra Bersanetti, Paul F. Alewood, Paul Heiss, Paul P. Masci, Paulo C. Carvalho, Pedro Gabriel Nachtigall, Pedro I. Silva, Pedro José Barbosa Pereira, Peter Roepstorff, Philippe Chan Tchi Song, Pierre Escoubas, Ping Chen, Piriya Wongkongkathep, Poorichaya Somparn, Qinan Wu, R. Manjunatha Kini, R.P. Rajesh, Rajesh R. Kannan, Rajesh Rajaian Pushpabai, Ramalingam Kirubagaran, Richard J. Lewis, Roderich D. Süssmuth, Rodrigo A.V. Morales, Rogério Lauria da Silva, Roman A. Zubarev, Ruchi P. Jain, Rui Cao, Rui Seabra Ferreira Júnior, Ruoxian Xv, Samira R. Aili, Samuel D. Robinson, Samuel S. Espino, Samuel Silva da Rocha Pita, Sanu Korumadathil Shaji, Sergio Marangoni, Shagufta Jahan, Siddhartha P. Sarma, Silvio S. Veiga, Simone König, Simone Vink, Sira Sriswasdi, Solange M.T. Serrano, Songping Liang, Stefanie K. Menzies, Stephen Earl, Sudarslal Sadasivan Nair, Suely G. Figueiredo, Suely Vilela Sampaio, Suman S. Thakur, Sunchai Payungporn, Sávio S. Sant’Anna, T. G. Galkina, Tania A. Nielsen, Tanner M. Schaub, Thalita Rocha, Thammakorn Saethang, Thiago F. Abreu, Thiago Verano-Braga, Thierry Jouenne, Thomas Crasset, Thomas F. Dyrlund, Tianbao Chen, Tobias Kessel, Tobias Wang, Trairak Pisitkun, Tristan P. Wallis, Tsezi A. Egorov, Tássia Rafaella Costa, Ursula C. Oliveira, V. E. Anisimova, Valentine Barassé, Valmir C. Barbosa, Valérie Troispoux, Victor Corasolla Carregari, Visith Sitprija, Vitor Vasconcelos, Waeowalee Choksawangkarn, Wei Zhang (Macau), Wenlong Zhang, Wolfgang M. J. Obermann, Xianbin Meng, Xianchun Wang, Xinping Xi, Yiguo Huo, Ying Wang, Yoshiaki Nakagawa, Yusuke Yoshimoto, Yuzo Yamazaki, Zhigui Duan, Zhihong Jiang, Zhonghua Liu

Where the work happened

Australia, Belgium, Brazil, Cabo Verde, Cameroon, China, Costa Rica, Denmark, Egypt, France, French Guiana, Germany, Guadeloupe, Hungary, India, Japan, Mexico, Myanmar, Portugal, Qatar, Saudi Arabia, Singapore, South Africa, Spain, Sweden, Thailand, Turkey, UK, USA

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